Determination of bisphenol A in beer by immunoaffinity column tandem high-performance liquid chromatography

KEYWORD: bisphenol A; immune-affinity column; high-performance liquid chromatography ABSTRACT: A rapid and sensitive method using immuno-affinity columns (IACs) as sample purification procedure and high-performance liquid chromatography (HPLC) tandem fluorescence detector (FLD) for detection has been developed for the determination of bisphenol A (BPA) in beer. The limit of detection (LOD) is 0.28 ng/ml (S/N=3) and the limit of quantity (LOQ) is 0.95 ng/ml (S/N=10). For standard adding and recovery experiments toward beer sample with addition of fourlevel bisphenol A, the average recovery ratio range from 74.56% to 97.60%, and the relative standard deviations (RSD) range 1.04% to 9.77%. In conclusion, this method is an appropriate way for detection of BPA. For the detection of canned beer samples of various brands, the detection rate is as high as 100%.